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ANNUAL PROGRESS REPORT
For the Period
September 1, 1994 to August 31, 1995
January 1996


North Central Regional Aquaculture Center
13 Natural Resources Building
Michigan State University
East Lansing, MI 48824-1222
Telephone: (517) 353-1962 FAX: (517) 353-7181


TABLE OF CONTENTS

INTRODUCTION 1
ORGANIZATIONAL STRUCTURE 2
ADMINISTRATIVE OPERATIONS 3
PROJECT DEVELOPMENT4
PROJECT REPORTING 6
PROJECT TERMINATION OR PROGRESS REPORTS 9
Extension (Progress Report) 11
Economics and Marketing (Progress Report) 15
Yellow Perch (Progress Report) 21
Hybrid Striped Bass (Progress Report) 29
Walleye (Project Component Termination Report) 33
Walleye (Progress Report) 41
Sunfish (Project Component Termination Report) 51
Sunfish (Progress Report) 55
Salmonids (Progress Report) 63
Crayfish (Termination Report) 69
Effluents (Termination Report) 73
Aquaculture Drugs (INADs/NADAs) (Progress Report) 79
APPENDIX (Publications, Manuscripts, Papers Presented) 83
Extension 85
Economics and Marketing 87
Yellow Perch 90
Hybrid Striped Bass 93
Walleye 94
Sunfish 100
Salmonids 101
Crayfish 102
Effluents 103
Aquaculture Drugs (INADs/NADAs) 104

INTRODUCTION

The U.S. aquaculture industry continues to be one of the fastest growing sectors within U.S. agriculture, although at a lesser rate than what occurred during the 1980s. Production in 1990 reached 861 million pounds and generated approximately $762 million for producers. The impact of U.S. aquaculture in 1990 was substantial: final sales value totalled $4.75 billion; direct and indirect economic impact was estimated to be $8.0 billion. Yet, anticipated growth in the industry, both in magnitude and in species diversity, continues to fall short of expectations.

Much of what is known about aquaculture science is a result of institutional attention given to our traditional capture of wild fisheries with the goal of releasing cultured fishes into public waters for enhancement of declining public stocks. Despite extensive efforts to manage wild populations for a sustained yield, as a nation we consume substantially greater amounts than we produce. Much of the United States' demand for seafood has been met by imports. The U.S. imports over 40% of its fish and shellfish and, after Japan, is the world's second largest importer of seafood. Fisheries imports---some $10.6 billion per year---are the largest contributor to the U.S. trade deficit among agricultural products, and third largest overall after petroleum and autos. The value of imported fisheries products more than doubled during the 1980s. In 1993, the trade deficit was $3.7 billion for all fisheries products, $2.8 billion of which was for edible fish and shellfish. In fact, foreign-grown aquaculture products constitute some $800 million of our fisheries imports.

Landings for most commercial capture fisheries species and recreational fisheries of the United States have been relatively stable during the last decade, with many fish stocks being overexploited. In this situation, aquaculture provides an opportunity to reduce the trade deficit and meet the rising U.S. demand for fish products. A strong domestic aquaculture industry is needed to increase U.S. production of fish and shellfish. This can be achieved by a partnership among the Federal Government, State and local public institutions, and the private sector with expertise in aquaculture development.

Congress recognized the opportunity for making significant progress in aquaculture development in 1980 by passage of the National Aquaculture Act (P.L. 96-362). Congress amended the National Agricultural Research, Extension, and Teaching Policy Act of 1977 (P.L. 95-113) in Title XIV of the Agriculture and Food Act of 1981 (P.L. 97-98) by granting authority to establish aquaculture research, development, and demonstration centers in the United States in association with colleges and universities, State Departments of Agriculture, Federal facilities, and non-profit private research institutions. Five such centers have been established: one in each of the northeastern, north central, southern, western, and tropical/subtropical Pacific regions of the country. The 1990 Farm Bill (Food, Agriculture Conservation, and Trade Act of 1990 - P.L. 101-624) has reauthorized the Regional Aquaculture Center program at $7.5 million per annum. As used here, a center refers to an administrative center. Centers do not provide monies for brick-and-mortar development. Centers encourage cooperative and collaborative aquaculture research and extension educational programs that have regional or national application. Center programs complement and strengthen other existing research and extension educational programs provided by the U.S. Department of Agriculture (USDA) and other public institutions. As a matter of policy, centers implement their programs by using institutional mechanisms and linkages that are in place in the public and private sector.

The mission of the Regional Aquaculture Centers (RACs) is to support aquaculture research, development, demonstration, and extension education to enhance viable and profitable U.S. aquaculture production which will benefit consumers, producers, service industries, and the American economy.

The North Central Regional Aquaculture Center (NCRAC) was established in February 1988. It serves as a focal point to assess needs, establish priorities, and implement research and extension educational programs in the twelve state agricultural heartland of the United States which includes Illinois, Indiana, Iowa, Kansas, Michigan, Missouri, Minnesota, Nebraska, North Dakota, Ohio, South Dakota, and Wisconsin. NCRAC also provides coordination of interregional and national programs through the National Coordinating Council for Aquaculture (NCC). The council is composed of the RAC directors and USDA aquaculture personnel.

ORGANIZATIONAL STRUCTURE

Michigan State University (MSU) and Iowa State University (ISU) work together to develop and administer programs of NCRAC through a memorandum of understanding. MSU is the prime contractor for the Center and has administrative responsibilities for its operation. The Director of NCRAC is located at MSU. ISU shares in leadership of the Center through an office of the Associate Director who is responsible for all aspects of the Center's publications, technology transfer and outreach activities.

At the present time the staff of NCRAC at MSU includes Ted R. Batterson, Director and Liz Bartels, Executive Secretary. The Center Director has the following responsibilities:

  • Serving as executive secretary to the Board of Directors, responsible for preparing agenda and minutes of Board meetings;
  • Serving as an ex-officio (non-voting) member of the Technical Committee and Industry Advisory Council;
  • Coordinating the development of research and extension plans, budgets, and proposals;
  • Coordinating and facilitating interactions among the Administrative Center, Board of Directors, Industry Advisory Council, and Technical Committee;
  • Monitoring research and extension activities;
  • Arranging for review of proposals for technical and scientific merit, feasibility, and applicability to priority problems and preparing summary budgets and reports as required;
  • Recruiting other Administrative Center staff as authorized by the Board of Directors;
  • With assistance of the Economics and Marketing Work Group, Technical Committee, or others preparing a summary of regional aquaculture, including production statistics and sales, and identifying technical, financial, and institutional constraints to expanding production. The summary shall include sections addressing established industries, development industries, and opportunities for new product development, and recommended research needs;
  • Maintaining liaison with other RACs; and
  • Serving on the NCC.


At the present time the staff of NCRAC's Office for Publications and Extension Administration at ISU includes Joseph E. Morris, Associate Director and Glenda Dike, Secretary. The Associate Director has the following responsibilities:

  • Serving as head of Publications for NCRAC, including editor of the NCRAC Journal, the newsletter of the Center;
  • Serving as the NCRAC liaison with national aquaculture extension programs, including in particular, extension programs of the other four USDA RACs; and
  • Serving as a member of NCRAC's Extension Executive Committee.


The Board of Directors (BOD) is the primary policy-making body of the NCRAC. The BOD has established an Industry Advisory Council (IAC) and Technical Committee (TC). Membership of the BOD consists of two persons from the IAC (the chair and an at-large member), a representative from the region's State Agricultural Experiment Stations and Cooperative Extension Services, a member from a non-land grant university and representatives from the two universities responsible for the center: Michigan State and Iowa State. The IAC is composed of representatives from each state's aquaculture association and six-at-large members appointed by the BOD who represent various sectors of the aquaculture industry and the region as a whole. The TC is composed of a sub-committee for Extension (TC/E) and a subcommittee for Research (TC/R). Directors of the Cooperative Extension Service within the North Central Region appoint representatives to the TC/E. The TC/R has broad regional make-up and is composed of scientists from universities and state agencies with varied aquacultural expertise who are appointed by the BOD. Each sub-committee of the TC has a chairperson who serves as an ex-officio member of the BOD.

NCRAC functions in accordance with its Operation Manual which is periodically amended and updated with BOD approval. It is an evolving document that has changed as the Center's history lengthens. It is used for the development of the cooperative regional aquaculture and extension projects that NCRAC funds.

ADMINISTRATIVE OPERATIONS

Since inception of NCRAC February 1, 1988, the role of the Administrative Center has been to provide all necessary support services to the BOD, IAC, TC, and project work groups for the North Central Region as well as representing the region on the NCC. As the scope of the NCRAC programs expand, this has entailed a greater work load and continued need for effective communication among all components of the Center and the aquaculture community.

The Center functions in the following manner.

  • After BOD approval of Administrative Center costs, the Center submits a grant to USDA/CSREES/Grants Management Branch for approval. To date the Center has received eight grants from USDA for FY88 (Grant #88-38500-3885), FY89 (Grant #89-38500-4319), FY90 (Grant #90-38500-5008), FY91 (Grant #91-38500-5900), FY92 (Grant #92-38500-6916), FY93 (Grant #93-38500-8392), FY94 (Grant #94-38500-0048), and FY95 (Grant #95-38500-1410) with monies totalling $5,680,501. Currently, five grants are active (FY91-95); the first three grants (FY88-90) have terminated.
  • The Center annually coordinates a program planning meeting which sets priorities for the next funding cycle and calls for regional workshops to develop project outlines to address priority problem areas.
  • Work Groups, which are formed at the workshops, submit project outlines to the Center. The projects are peer reviewed by experts from both within and outside the region.
  • The BOD, using reviewers' responses, decide which projects are to be approved and funding levels. The Center conveys BOD decisions to all Project Work Groups. Those that are approved for funding are asked to submit revised project outlines incorporating BOD and reviewers' comments.
  • The Center then submits the revised project outlines as a Plan of Work (POW) to USDA for approval.
  • Once a POW is approved by USDA, the Center then prepares subcontracts for each participating institution. The Center receives all invoices for subcontractual agreements and prepares payment vouchers for reimbursement. Thus, the Center staff serve as fiscal agent for both receiving and disbursing of funds in accordance with all terms and provisions of the grants.


To date, the Center has funded or is funding 36 projects through 207 subcontracts from the eight grants received. Funding for all Center supported projects, except for Publications and a development of an Aquaculture Situation and Outlook Report, is summarized in Table 1 below (pages 7-8).

During this reporting period, the Publications Office at ISU produced and distributed a number of publications including fact sheets, technical bulletins, videos, and two issues of the Centers newsletter, the NCRAC Journal. A complete list of all publications from this office is included in the Appendix under Extension.

Other areas of support by the Administrative Office during this reporting period included: monitoring research and extension activities and developing progress reports; preparing a compendium progress report for all five RACs; developing liaisons with appropriate institutions, agencies and clientele groups; preparing, in coordination with the other RACs, both written and oral testimony for the U.S. House Appropriations subcommittee on Agriculture, Rural Development, Food and Drug Administration, and Related Agencies hearing in Washington, D.C.; participating in the NCC; numerous oral and written presentations to both professional and lay audiences; and working with other fisheries and aquaculture programs throughout the North Central Region.

PROJECT DEVELOPMENT

A joint Program Planning meeting of the BOD, IAC, and TC is held every year in the early winter. The IAC, with input from the TC, generates a list of priority areas for consideration by the BOD. Using their recommendation as guidelines, the BOD then selects priority areas for which project outlines will be developed. The BOD also specifies a maximum funding level for each priority area. Problem statements and objectives are then developed for each priority area by IAC and TC members at the Program Planning meeting. For projects with more than one objective, the IAC ranks the objectives by priority. The problem statement and objective(s) are then included in a workshop announcement that is broadly distributed throughout the North Central Region. The workshops are one-day events to establish a work group that will develop a project outline over the summer months. Work group members will be those who have demonstrated that they have the expertise and facilities for undertaking the proposed work in regard to a particular objective or objectives. The proposed work cannot deviate from the objective or objectives included in the workshop announcement. The work group elects a chair and secretary. The chair is responsible for submitting the project outline to the NCRAC Director; the secretary is responsible for preparing minutes from the workshop that are distributed to all attendees. All project outlines are peer reviewed. The reviewers' comments are used by the BOD in making the final selection of projects and level of funding at the following year's annual Program Planning meeting. All work group members are apprised of the BOD decisions. Revisions of projects approved by the BOD are submitted by the work group chair to the NCRAC Director. The revised project outlines are then included in a POW that is submitted to USDA. Upon approval by USDA, the Center issues subcontracts to the funded work group members.

TIME FRAME

Program Planning meeting: early winter.

Workshops: late-spring, early summer.

Project outlines developed over the summer by work group members who participated in the workshops. These project outlines are then submitted to the Center in the fall and peer reviewed.

The Board of Directors at the following year's Program Planning meeting selects the projects to be funded.

Project outline revised and submitted to the Center by May.

Revised projects are then submitted in June as a POW to USDA for approval. Once approved by USDA subcontracts are let by the Center with a start date of September 1.

By following this procedure, it takes approximately 18 months from the time of identifying a priority area until inception of a project to address the issue in question.

WORKSHOPS

The purpose of the workshops is to bring together those who are best qualified to work on project objectives by virtue of a demonstrated record of expertise and access to facilities required in the project. These people form a work group for the purpose of writing a project outline to address the problem in question. The following criteria typically apply to those projects that are funded by NCRAC.

Involves participation by two or more states in the North Central Region;

requires more scientific manpower, equipment, and facilities than generally available at one location;

approach is adaptable and particularly suitable for inter-institutional cooperation resulting in better use of limited resources and a saving of funds;

will complement and enhance ongoing extension and research activities by participants, as well as offer potential for expanding these programs;

is likely to attract additional support for the work which is not likely to occur through other programs and mechanisms;

is sufficiently specific to promise significant accomplishments in a reasonable period to time (usually up to 2 years);

can provide the solution to a problem of fundamental importance or fill an information gap;

can be organized and conducted on a regional level, assuring coordinated and complementary contributions by all participants.

The NCRAC program pays no overhead to participating institutions nor tuition remission, has no brick-and-mortar money, and relies on in-place salaried personnel, equipment, and facilities to carry out the projects. Due to the collaborative and cooperative nature of these regional projects, no one individual or institution receives a significant portion of the total project funds.

PROJECT REPORTING

As indicated in Table 1, the Center has funded a number of projects for many of the project areas. For example, there have been five separately funded projects in regard to Extension and six for Walleye. Project outlines have been written for each separate project within an area, or the project area itself if only one project. These project outlines have been submitted in POWs or amendments to POWs for the grants as indicated in Table 1. Many times, the projects within a particular area are merely continuations of previously funded activities; while at other times they are addressing new objectives. Presented below are Progress or Termination Reports for all projects that were underway or completed during the period September 1, 1994 to August 31, 1995.

All publications, manuscripts, or papers for the different project areas are listed in the Appendix.

Table 1. North Central Regional Aquaculture Center funded projects.

Project Area Project

Number

Duration Funding Level Grant Number
Extension 1

2

3

4

5

5/1/89-4/30/91

3/17/90-8/31/91

9/1/91-8/31/93

9/1/93-8/31/95

9/1/95-8/31/97

$39,221

$68,389

$94,109

$110,129

$14,275

$25,725


$351,848

88-38500-3885

89-38500-4319

91-38500-5900

91-38500-5900

92-38500-6916

95-38500-1410

Economics and Marketing 1

2

3

5/1/89-12/31/91

9/1/91-8/31/93

9/1/93-8/31/95

$127,338

$34,350

$53,300

$40,000


$254,988

88-38500-3885

89-38500-4319

91-38500-5900

93-38500-8392

Yellow Perch 1

2

3

4

5

5/1/89-8/31/91

6/1/90-8/31/92

9/1/91-8/31/93

9/1/93-8/31/95

9/1/95-8/31/97

$76,957

$85,723

$92,108

$99,997

$150,000

$200,000


$704,785

88-38500-3885

89-38500-4319

90-38500-5008

91-38500-5900

93-38500-8392

95-38500-1410

Hybrid Striped Bass 1

2

3

4

5

5/1/89-8/31/91

6/1/90-8/31/92

9/1/91-8/31/93

9/1/93-8/31/95

9/1/95-8/31/97

$68,296

$68,114

$101,000

$96,550

$168,000

$160,000


$661,960

88-38500-3885

89-38500-4319

90-38500-5008

91-38500-5900

93-38500-8392

95-38500-1410

Walleye 1

2

3

4

5

6

5/1/89-8/31/91

6/1/90-8/31/92

9/1/91-8/31/92

9/1/92-8/31/93

9/1/93-8/31/95

9/1/95-8/31/97

$177,517

$111,657

$109,223

$75,000

$150,000

$117,897

$57,103


$798,397

89-38500-4319

90-38500-5008

91-38500-5900

89-38500-4319

93-38500-8392

94-38500-0048

95-38500-1410

Sunfish 1

2

3

6/1/90-8/31/92

9/1/92-8/31/94

9/1/94-8/31/96

$130,758

$149,867

$174,999


$455,624

90-38500-5008

92-38500-6916

94-38500-0048

Salmonids 1

2

3

6/1/90-8/31/92

9/1/92-8/31/94

9/1/94-8/31/96

$9,000

$120,799

$149,997

$200,000


$479,796

89-38500-4319

90-38500-5008

92-38500-6916

94-38500-0048

NCR Aquaculture Conference 1 6/1/90-12/31/91 $7,000 90-38500-5008
National Aqua. Extension Workshop 1 10/1/91-9/30/92 $3,005 89-38500-4319
Crayfish 1 9/1/92-8/31/94 $50,000 92-38500-6916
Baitfish 1 9/1/92-8/31/94 $62,000 92-38500-6916
Effluents 1 9/1/92-8/31/94 $153,300 92-38500-6916
Aquaculture Drugs (INADs/NADAs) 1 9/1/93-8/31/94

9/1/94-8/31/95

$2,000

$5,000


$7,000

89-38500-4319

94-38500-0048

PROJECT TERMINATION OR PROGRESS REPORTS EXTENSION

Progress Report for the Period

May 1, 1989 to August 31, 1995

NCRAC FUNDING LEVEL: $311,848 (May 1, 1989 to August 31, 1995)

PARTICIPANTS:

Fred P. Binkowski University of Wisconsin-Milwaukee Wisconsin
James E. Ebeling Ohio State University Ohio
Donald L. Garling Michigan State University Michigan
Jeffrey L. Gunderson University of Minnesota Minnesota
F. Robert Henderson Kansas State University Kansas
Anne R. Kapuscinski University of Minnesota Minnesota
Terrence B. Kayes University of Nebraska-Lincoln Nebraska
Ronald E. Kinnunen Michigan State University Michigan
Christopher C. Kohler Southern Illinois University-Carbondale Illinois
David J. Landkamer University of Minnesota Minnesota
Joseph E. Morris Iowa State University Iowa
Kenneth E. Neils Kansas State University Kansas
Robert A. Pierce II University of Missouri Missouri
Daniel A. Selock Southern Illinois University-Carbondale Illinois
LaDon Swann Purdue University Indiana/Illinois
Administrative Advisor:    
David C. Petritz Purdue University Indiana







PROJECT OBJECTIVES

(1) Strengthen linkages between North Central Regional Aquaculture Center (NCRAC) research and extension work groups.

(2) Enhance the North Central Region (NCR) aquaculture extension network for aquaculture information transfer.

(3) Provide in-service training for Cooperative Extension Service (CES) and Sea Grant personnel and other landowner assistance personnel.

(4) Develop and implement aquaculture education programs for the NCR.

(5) Participate in development of NCRAC publications.

ANTICIPATED BENEFITS

The NCRAC Extension Work Group will promote and advance commercial aquaculture in a responsible fashion through an organized education/training outreach program. The primary benefits will be:

increased public awareness through publications, short courses, and conferences regarding the potential of aquaculture as a viable agricultural enterprise in the NCR;

technology transfer to enhance current and future production methodologies for selected species, e.g., walleye, hybrid striped bass, yellow perch, salmonids, and sunfish, through hands-on workshops and field demonstration projects;

improved lines of communication between interstate aquaculture extension specialists and associated industry contacts; and

enhanced legal and socioeconomic atmosphere for aquaculture in the NCR.

PROGRESS AND PRINCIPAL ACCOMPLISHMENTS

OBJECTIVE 1

Due to the efforts of aquaculture extension personnel in the NCR, NCRAC's Board of Directors formally adopted guidelines for Extension's involvement in all Center funded projects. These guidelines integrate research and extension activities so that Extension service personnel can better serve their clientele groups.

In addition, aquaculture Extension Work Group members have:

Helped conduct a survey of crayfish producers in the NCR and completed a report on Orconectes immunis for inclusion in the Crayfish Work Group report.

Provided the NCRAC Economics and Marketing Work Group with information relevant to that group's efforts to develop cost of production budgets and expected revenues for the commercial production of food-sized hybrid striped bass, walleye, and yellow perch in the NCR.

Assisted in writing and developing the NCRAC Walleye Culture Manual that is being edited by Bob Summerfelt of Iowa State University.





OBJECTIVE 2

The demand for aquaculture extension education programs cannot be met by the few specialists in the NCR (4.0 FTE). Networking of specialists and CES designated contacts has maximized efficiency of education programs and minimized duplication. The NCRAC Extension Project is designed to assess and meet the information needs of the various clientele groups through cooperative and coordinated regional educational programming.

Aquaculture handbooks have been developed and distributed to each NCRAC designated aquaculture extension specialist and selected CES and Sea Grant field staff.

As with any organization, there have been changes in NCRAC extension personnel since the inception of the project. Landkamer was the primary aquaculture extension contact for Minnesota. However, he left the university and Kapuscinski became the primary contact person. Recently, Gunderson has assumed that responsibility. Two other individuals, who had served since the outset of the project as their state's aquaculture extension contact, were replaced in 1994. In Kansas, Neils replaced Henderson and in Illinois, Kohler replaced Selock.

OBJECTIVE 3

In-service training for CES and Sea Grant personnel and other landowner assistance personnel have been held in most of the states in the region. Training has been in the areas of basic aquaculture and safe seafood handling including HACCP (Hazard Analysis Critical Control Point).





OBJECTIVE 4

A number of workshops, conferences, videos, field-site visits, hands-on training sessions, and other educational programs have been developed and implemented.

There have been workshops on general aquaculture, fish diseases, commercial recirculation systems, aquaculture business planning, crayfish culture, pond management, yellow perch and hybrid striped bass culture, rainbow trout production, and polyploid induction in sunfish held in the region.

Two North Central Aquaculture Conferences (NCAC) have been held. The first in Kalamazoo, Michigan was held in March 1991. The second was held in February 1995 in Minneapolis, Minnesota. These regional meetings were attended by hundreds of individuals including persons from Canada.

On April 10, 1993, over 700 viewers from 35 states and Canada watched the first national interactive teleconference on aquaculture, "Investing in Freshwater Aquaculture" that was broadcast from Purdue University. It was a televised satellite broadcast for potential fish farmers. The program consisted of 10 five- to seven-minute video tape segments which addressed production aspects of channel catfish, crayfish, rainbow trout, hybrid striped bass, tilapia, yellow perch, baitfish, and sportfish. A set of course materials was available prior to the program. Three times during the program, a question and answer period was available to the audience through a toll free telephone number. Questions not answered during the program were answered by mail afterwards. The entire teleconference is available as a videotape from NCRAC's Publications Office as well as two other videotapes by the University of Nebraska-Lincoln that are reprises of the broadcast.

OBJECTIVE 5

Numerous fact sheets, technical bulletins, and videos have been written or produced by various participants of the Extension Work Group. These are listed in the Appendix.

WORK PLANNED

Efforts will continue in regard to strengthening linkages between research and extension work groups as well as enhancing the network for aquaculture information transfer. Participants will also continue to provide in-service training for CES, Sea Grant, and other land-owner assistance personnel. Educational programs and materials will be developed and implemented. This includes a workshop on hybrid striped bass (proceedings and videos), a walleye culture manual, yellow perch culture guide and videos, a marketing video for aquaculture products, and a production guide and videos about sunfish.

Additional workshops developed and hosted by state extension contacts will be advertised in surrounding states to take advantage of the NCRAC extension network and the individual expertise of Extension Work Group participants.

Several NCRAC fact sheets, technical bulletins, and videos will be developed by various Work Group members.

IMPACTS

In-service training for CES and Sea Grant personnel has enabled those professionals to respond to initial, routine aquaculture questions from the general public.

Development of aquaculture education programs for the NCR has provided "hands-on" opportunities for prospective and experienced producers. Approximately 5,000 individuals have attended workshops or conferences organized and delivered by the NCRAC Extension Work Group. Clientele attending regional workshops learned of aquaculture development strategies in other areas of the country and acquired information which was of direct use to their own enterprises. Education programs also created situations where problems encountered by producers were expressed to extension personnel who later relayed them to researchers at NCRAC work group meetings for possible solutions through the research effort.

Fact sheets, technical bulletins, and videos have served to inform a variety of clients about numerous aquaculture practices for the NCR. For instance, "Making Plans for Commercial Aquaculture in the North Central Region" is often used to provide clients with initial information about aquaculture, while species specific publications on walleye, trout, and catfish have been used in numerous regional meetings and have been requested by clients from throughout the United States. Publications on organizational structure for aquaculture businesses, transportation of fish in bags, and others are beneficial to both new and established aquaculturists. In a 1994 survey, NCRAC extension contacts estimated that NCRAC publications were used to address approximately 15,000 client questions annually.

NCRAC extension outreach activities have helped to foster a better understanding and awareness for the future development of aquaculture in the region.

PUBLICATIONS, MANUSCRIPTS, WORKSHOPS, AND CONFERENCES

See Appendix.

SUPPORT

YEARS NCRAC-USDA FUNDING OTHER SUPPORT TOTAL

SUPPORT

UNIVER-SITY INDUSTRY OTHER

FEDERAL

OTHER TOTAL
1989-90 $39,221 $66,992       $66,992 $106,213
1990-91 $68,389 $70,065       $70,065 $138,454
1991-93 $94,109 $152,952       $152,952 $247,061
1993-95 $110,129 $198,099   $250,000 $55,000 $503,099 $613,228
TOTAL $311,848 $488,108   $250,000 $55,000 $793,108 $1,104,956

ECONOMICS AND MARKETINGProgress Report for the Period

September 1, 1993 to August 31, 1995

NCRAC FUNDING LEVEL: $40,000 (September 1, 1993 to August 31, 1995)

PARTICIPANTS:

Susan B. Kohler Southern Illinois University-Carbondale Illinois
Marshall A. Martin Purdue University Indiana
Patrick D. O'Rourke Illinois State University Illinois
Jean R. Riepe Purdue University Indiana
Extension Liaisons:    
Donald L. Garling Michigan State University Michigan
Terrence B. Kayes University of Nebraska-Lincoln Nebraska
Daniel A. Selock Southern Illinois University-Carbondale Illinois
LaDon Swann Purdue University Indiana






PROJECT OBJECTIVE

Develop cost of production budgets and expected revenues for the raising of food-sized walleye, yellow perch, and hybrid striped bass on farms in the North Central Region (NCR).

ANTICIPATED BENEFITS

The overall goal of this collaborative project is to enhance walleye, yellow perch and hybrid striped bass production by developing enterprise budgets for production of these species in the NCR. This supports the mission of NCRAC, especially by conducting research "for the enhancement of viable and profitable commercial aquacultural production in the United States for the benefit of producers, consumers, and the American economy."

The cost of production or budgeting components of this project offers the potential to help in identifying production systems for walleye, yellow perch and hybrid striped bass which are most likely to be commercially viable. Information on production costs is quite limited for these species, especially walleye and yellow perch. Enterprise budgets will enable producers to access the needed budget costs for comparisons for their own operation, for a new enterprise, or for increased production in their present facility in comparison to reasonable expectations about market prices.

This project will benefit the aquaculture industry in the NCR in several ways, even though there are some limitations of these budgets given the "emerging" status of the industry and given the nature of budgets.

First, objectively developed cost information is typically more accurate than subjectively developed cost information or no information on costs at all. These budgets will give producers an idea of how enterprise budgets should be organized, what types of data need to be collected, and why good record keeping is essential. The production values and relationships upon which the cost structure is based, while not standardized in the industry, should serve as a rough rule-of-thumb by which aquacultural producers can gauge their management skills.

Second, enterprise budgets are an excellent management tool for producers. If the publication of these budgets stimulates potential and current aquacultural producers to put together budgets that reflect their unique enterprises, then the industry will be much better off.

Third, enterprise budgets are the cornerstone for all different types of financial analysis of aquaculture operations. The budgets will allow more economic research into financial aspects of aquaculture and enable those producers who are spurred on to put together budgets to push on into their own financial analysis (another management tool).

Fourth, enterprise budgets are also the cornerstone for sensitivity analysis (yet another management tool). Undertaking sensitivity analysis will enable economists and producers to better understand the relative importance of cost and production items in the budget and to explore the boundaries of enterprise profitability.

Finally, realizing that the budgets produced under the auspices of this project will not be the final, definitive budgets for aquaculture production in the NCR, they will serve as a solid starting base from which to update information and expand into alternative species, production systems, life stages, etc.

In a more indirect way, the enterprise budgets will accomplish two other important things.

One, the budgets may help guide research and extension decisions by NCRAC work group participants, the Industry Advisory Council, the Board of Directors, and the supporting committees.

Second, the budgets will provide an opportunity for the economists and other personnel developing the budgets to interact with aquaculture producers, researchers, and extension personnel in the NCR. This type of interdisciplinary interaction is vital for the improved understanding and communication of all vital aspects of aquaculture in the NCR.

Economic feasibility analysis will help producers evaluate technical advances in fish production. This contribution is critical as a guide to future research funding in the various species and production systems suitable for commercial production. The distribution of research results from this project through the publications of the Economics and Marketing Work Group and through the Extension Liaisons using computer budget software will provide a structured and informed dissemination system which is credible with producers, financial institutions and others.

PROGRESS AND PRINCIPAL ACCOMPLISHMENTS

HYBRID STRIPED BASS

Kohler has compiled a review of the literature on hybrid striped bass (HSB) production and production costs. The literature reviewed is summarized in an annotated bibliography. This bibliography will be available to anyone needing the information.

HSB cost of production estimates were developed from six published reports on HSB production. These estimated costs will be presented at the NCRAC HSB Workshop in November 1995 and will be submitted for possible release as an NCR publication.

WALLEYE

O'Rourke and Illinois State University (ISU) graduate students have completed an extensive walleye production/culture literature review. The primary focus of the literature review was to find any research findings that might be useful in ascertaining the cost of production for walleye fingerlings and food-sized fish under intensive and extensive culture regimes. Very little economic research was found and even less was found that was documented well enough to be useful.

The second source of information was a survey of research experts and hatchery personnel familiar with walleye culture. The ISU investigators were surprised that many of the "experts" were as reluctant to share information as were most entrepreneurs/producers. The experts were selected and queried using a modified Delphi approach for both the fingerling and food-sized studies. This stage of the research was completed in 1995 with additional follow-up questions and identification of a broader group of "experts."

Work has advanced on identifying and analyzing the cost of production for advanced walleye fingerlings and food-sized walleye in intensive and extensive culture systems. The first Master's thesis was finished in December 1994. It is an economic feasibility analysis of a tank based intensive walleye fingerling production system. The second Master's thesis, an economic feasibility analysis of a tank based intensive food-sized walleye system, was finished in August 1995.

YELLOW PERCH

Riepe submitted a manuscript to NCRAC for publication as an extension technical bulletin. This publication reports the results of her research into the costs of culturing yellow perch. The manuscript is based on cost estimates for growing out advanced fingerlings within one growing season. Cost estimates were generated for two types of production systems: cages and levee ponds; and two sizes of operations: 2,268 kg (5,000 lb) and 22,680 kg (50,000 lb).

Sensitivity analysis also was conducted to test the impact of alternative budget parameters (production values and individual cost items) on the overall break-even price. A Master's graduate student was assigned to conduct research in costs of producing perch in recirculating tank systems and a thesis was completed in 1995. Costs of growing out yellow perch in recirculating tank systems were analyzed for two sizes of operations: 1,588 kg (3,500 lb) and 2,268 kg (5,000 lb).

While developing cost estimates for yellow perch aquaculture, Riepe investigated feed and fingerling prices and procurement with various suppliers. Riepe used this information to develop tables estimating delivered prices for feed and fingerlings at five hypothetical producer locations around the North Central Region.

Following the technical bulletin, Riepe developed a fact sheet focusing on pond production costs and a fact sheet focusing on managing feed costs. The fact sheet on costs of pond production was a simplified, extension version of the pond enterprise budgets and discussion included in the technical bulletin. The fact sheet on managing feed costs was developed based on Riepe's conversations with feed manufacturers as well as the price and transportation data they provided.

WORK PLANNED

The distribution of research results from this project through various publications and through the Extension Liaisons using computer budget software will provide a structured and informed dissemination system which is credible with producers, financial institutions and others.

Kohler will submit the annotated bibliography on hybrid striped bass for publication. The HSB production cost estimates, which were developed from the literature review and farm visits, will be presented at an NCRAC HSB workshop in November 1995.

O'Rourke will continue economic analysis of walleye fingerling and food-sized fish production systems. Results from the two Master's theses studies will be submitted for technical bulletin and/or fact sheet publication through NCRAC and professional presentations.

Riepe received comments back from NCRAC reviewers on all three submitted manuscripts in mid-October 1995. She plans to revise manuscripts and submit them for publication in October - December 1995.

IMPACTS

Extension Liaison Don Garling hosted a yellow perch aquaculture workshop in June

1995. The results of Riepe's work on perch production costs were presented at that workshop. Attendees indicated that they were considering the types of systems modeled by Riepe. O'Rourke presented the preliminary results of the work on walleye fingerling tank based system cost of production at the Combined North Central and Ninth Annual Minnesota Aquaculture Conference in February 1995. Finally, Kohler will present the results of the review of HSB production costs at the NCRAC Hybrid Striped Bass Workshop in November 1995.

The information developed and presented for the three species is anticipated to be directly useful to the attendees as they consider their own operations and intentions in light of the cost data presented.

This project has already benefited the aquaculture industry in the NCR through the workshop presentations. As a result of this project, economists have been able to develop and deliver presentations on economic issues in aquaculture production to current and potential aquacultural producers. These presentations and the publications which follow may reduce the impacts of uninformed investment decisions by current and potential aquaculture entrepreneurs.

PUBLICATIONS, MANUSCRIPTS, AND PAPERS PRESENTED

See Appendix.SUPPORT

YEARS NCRAC-USDA FUNDING OTHER SUPPORT TOTAL

SUPPORT

UNIVER-SITY INDUSTRY OTHER

FEDERAL

OTHER TOTAL
1993-94 $22,104 $35,829       $35,829 $57,933
1994-95 $17,896 $23,854       $23,854 $41,750
TOTAL $40,000 $59,683       $59,683 $99,683







YELLOW PERCH

Progress Report for the Period

September 1, 1993 to August 31, 1995

NCRAC FUNDING LEVEL: $150,000 (September 1, 1993 to August 31, 1995)

PARTICIPANTS:

Fred P. Binkowski University of Wisconsin-Milwaukee Wisconsin
Paul B. Brown Purdue University Illinois
Konrad Dabrowski Ohio State University Ohio
Terrence B. Kayes University of Nebraska-Lincoln Nebraska
Jeffrey A. Malison University of Wisconsin-Madison Wisconsin
Extension Liaison:    
Donald L. Garling Michigan State University Michigan
Non-funded Collaborators:  
Harlan Bradt, etc. Coolwater Farms, LLC, Cambridge Wisconsin
William Hahle Pleasant Valley Fish Farm, McCook Nebraska
John Hyink/John Wolf Alpine Farms/Glacier Springs Trout Hatchery Wisconsin
Dave Smith Freshwater Farms of Ohio, Inc., Urbana Ohio
Michael Wyatt Sandhills Aquafarm, Keystone Nebraska






PROJECT OBJECTIVES

(1) Determine the commercial scale feasibility and improve on the best intensive tank and pond culture practices for the production of yellow perch fingerlings.

(2) Determine the commercial scale feasibility of raising food-size yellow perch in flow-through raceways or tanks, open ponds, and large net-pens, comparing the best available formulated diets.

ANTICIPATED BENEFITS

At the 1992 Program Planning Meeting of the North Central Regional Aquaculture Center (NCRAC), the NCRAC Industry Advisory Council advanced the position that the primary emphasis of research projects proposed for the 1993-1995 funding period should be on the demonstration of commercial-scale feasibility of the best available research-based production technologies, working in collaboration with private fish producers. The NCRAC Board of Directors supported that position, and the NCRAC Yellow Perch Work Group responded by developing a project centered largely on conducting such demonstrations. This project is aimed at providing much needed information on the practical feasibility and costs of employing, on a commercial-scale, selected fingerling production and grow-out strategies that were initially developed and/or tested on a small (laboratory) scale. In addition, this project will develop improved technologies for certain key facets of yellow perch aquaculture. Finally, the results of experiments incorporated into this project will immediately help fish farmers improve the production efficiency of both fingerling and food-size yellow perch.



PROGRESS AND PRINCIPAL ACCOMPLISHMENTS

As an integral component of much of this project, private producers were to provide the requisite facilities, fish, feed, day-to-day husbandry, and routine data collection. At its inception, this project included the participation of eight different private fish farms in various parts of the North Central Region (NCR). Participating university researchers were to provide project oversight on experimental design, advice or direct assistance with the technical set-up of any specialized experimental systems, supervision and assistance on critical end-point data collection, and analyses of results.

In Year 1 of the project (September 1, 1993 to August 31, 1994), significant progress was made at certain sites at testing selected research-based production technologies. Accordingly, from an extension perspective, the project is successfully building and/or expanding working relationships between NCRAC researchers and certain regional fish farmers, testing various research-based technologies under practical production conditions, transferring knowledge from academia to the private sector, and identifying private producers who are both capable and willing to sustain a collaborative technology evaluation and demonstration effort. Several of the original private-sector collaborators have either met or have worked hard to meet their project commitments.

However, from the research perspective, the likelihood of the project generating significant amounts of comprehensive feasibility-assessment data that will be publishable in peer-reviewed journals seems low, based on Year 1 results. One primary reason for this development is that several private-sector collaborators who had initially agreed to participate in the project have either partially or totally pulled back from their original commitments. The reasons for these pullbacks range from initially exaggerated claims of facility and other resource capabilities, to lack of sufficient technical competence or experience, to a belated realization of the potential risks involved. To deal with this problem, in some instances alternate private sector collaborators were sought for studies conducted in Year 2 (September 1, 1994 to August 31, 1995), and in other cases feasibility assessments will be conducted under laboratory conditions rather than under field conditions as originally proposed.

OBJECTIVE 1

Researchers at the University of Wisconsin-Milwaukee (UW-Milwaukee) directed their efforts during the first year of the study at improving the intensive culture technology for yellow perch. In part, these results were applied to a commercial production operation using Recirculating Aquaculture Systems (RAS) technology.

In January 1995, Alpine Farms installed a 29,000 L (7,661 gal) Aqua-Manna type RAS and is using it to demonstrate the grow-out of fingerling perch to market size. In February 1995, this system was stocked with approximately 10,000 (50-75 mm; 1.97-2.95 in) yellow perch fingerlings (total 14 kg; 30.9 lb). Following stocking, some initial mortality occurred due to inadequate preconditioning and maturation of the biofiltration system and entrainment of small-sized fish on the clarifier filtration mechanism. A surplus of perch fingerlings was used to stock the tank to its original number of fish. Un-ionized ammonia levels during this period necessitated periodic water exchanges. By March, the fish were feeding and ammonia concentrations had stabilized as the biofiltration system matured. Mean water quality parameters (and range) from 228 days of operation were 22.0C (17.8 - 24.4; 71.6F, 64.0 - 75.9; N = 210), 6.0 mg dissolved oxygen/L (5.2 - 9.0, N = 207), pH 7.0 (6.1 - 8.1, N = 197), 0.003 mg/L NH3 (0 - 0.156, N = 194), and 0.165 mg/L NO2 (0 - 0.8, N = 93). Feeding was continually adjusted based on food acceptance and variations in water quality. Ration levels eventually stabilized at 1.0 to 2.0% of body weight. Perch were periodically subsampled from the RAS for growth and food conversion estimates. Overall growth in length was between 0.5 and 0.6 mm (0.020 and 0.024 in) per day as would be expected for perch reared at these temperatures. Overall food conversion was 0.9 kg feed (dry): kg perch gain (wet). Similar conversions have been obtained in other intensive flow-through tank rearing units with similar feeds. Further trials at higher densities are needed to fully evaluate the rearing performance of yellow perch in this type RAS system.

Studies on pond fingerling production by the University of Wisconsin-Madison (UW-Madison) were conducted using ponds of various sizes at Coolwater Farms, LLC and the Lake Mills State Fish Hatchery. These studies evaluated the use of (1) selected high fry stocking densities and early pond harvest to maximize pond fingerling production, and (2) underwater lights and vibrating feeders to habituate perch fingerlings to formulated feeds while they remain in ponds.

The production of 17-22 mm (0.67-0.87 in) total length (TL) fingerlings (the smallest size at which perch can be habituated to conventional starter feeds) not habituated to formulated feeds in ponds with high initial fry stocking rates (e.g., 1,000,000 fry/hectare; 404,700 fry/acre) averaged 375,000 fingerlings/ha (151,763 fingerlings/acre). Other studies showed that feed training of fingerlings at this size in tanks resulted in about 60% survival. Accordingly, the maximum production of feed-trained perch fingerlings using the best available tandem pond/tank methods was about 225,000 fingerlings/ha (375,000 × 60%) (91,058 fingerlings/acre).

The production of feed-trained fingerlings from ponds equipped with underwater lights and automatic feeders was as follows: (1) ponds stocked at 1,000,000 fry/ha (404,700 fry/acre) averaged 320,000 fingerlings/ha (129,504 fingerlings/acre) (i.e., a 32% return); (2) ponds stocked at 2,000,000 fry/ha (809,400 fry/acre) averaged 400,000 fingerlings/ha (161,880 fingerlings/acre) (i.e., a 20% return); and (3) ponds stocked at 3,000,000 fry/ha (1,214,000 fry/acre) averaged 76,000 fingerlings/ha (30,757 fingerlings/acre) (an average 2.5% return). The poor survival of fingerlings in ponds stocked at 3,000,000 fry/ha (1,214,000 fry/acre) apparently resulted from high starvation rates of fish prior to the habituation to feeding formulated feeds. This starvation, in turn, resulted from a decline in the natural forage base caused by excessive fish densities. All ponds were harvested shortly after large numbers of fish exhibited an aggressive feeding response, at which time the fingerlings were 30-45 mm (1.18-1.77 in) TL. Based on tests conducted in tanks, virtually all of the fingerlings harvested were habituated to formulated food. The wide size range among harvested fingerlings suggests that feeding methods and strategies have a profound effect on the survival and growth of fingerling perch habituated to formulated feeds in ponds.

To address this latter problem, UW-Madison investigators developed and began preliminary tests of an improved pond light and feeding system. The new system is designed to address the problems of reliability and limited feed distribution that were inherent with the original design. Initial indications are that the new system is durable, has a wide feed distribution pattern, and requires less manpower for operation and maintenance.

University of Nebraska-Lincoln (UNL) efforts in Year 2 were focused on pond-production field trials conducted at Pleasant Valley Fish Farm, McCook, Nebraska. Particular emphasis was placed on replicating and improving on results obtained in Year 1 from field trials aimed at enhancing procedures for the commercial-scale production of yellow perch fry and fingerlings - including the habituation of advance fry to formulated feed and grow-out to advanced fingerlings in ponds. About 200,000 (2,500,000/ha; 1,011,750/acre) eyed-eggs or fry were stocked into two 0.08-ha × 1.5-m-deep (0.20-acre × 4.92-ft-deep) rectangular ponds which are drainable and can be supplied with groundwater to moderate temperature extremes. Both ponds were fertilized during the early fry production period (about 3 weeks) and were continuously aerated as described in the original proposal. After that, automatic vibrating feeders equipped with underwater lights and controlled by an electronic timing system were added to the ponds to habituate the fish to a starter trout diet (Sterling Silver Cup, Sterling H. Nelson & Son, Inc., Murray, Utah).

Two changes in procedures from those used in Year 1 were: (1) the number of feeding stations in each pond was increased from five to seven; and (2) in one pond, some advanced fry were concentrated in a net-pen around one of the feeders. Spring and early summer in Nebraska in 1995 were characterized by unseasonably cold weather, much more so than 1994. To compensate in part for the difference between years, a decision was made to delay the harvest of the two ponds from late September (as in 1994) to late October (1995). Because of this, specific numerical findings on growth and other production parameters have not yet been fully analyzed, and therefore are not available for this report. However, visual observations suggest that the percentage of large versus small perch harvested was significantly improved by the addition of more feeders to each pond. Also, the perch concentrated in the net-pen appeared to habituate to feed much more readily than fish ranging free in the ponds. No field trial to habituate young perch to formulated feed in tanks was done at the Pleasant Valley Fish Farm in 1995 because of a lack of sufficient time and funds to set up the necessary tanks and feeding systems.

Following the procedure developed during the first year of research, Ohio State University (OSU) researchers manually spawned yellow perch brood stock (2 year olds) during the months of April, May, and June 1995. Due to an unseasonably warm winter and cold spring and perhaps nutritional deficiencies, the egg quality was very poor with extremely low hatching rates and survival. A limited number of eggs were incubated and eyed-staged embryos stocked out into two 0.1 ha (0.25 acre) research ponds on May 15, 1995 at a density of approximately 100,000 embryos per pond. The ponds were fertilized weekly with 3 L (0.79 gal) 28-0-0 and 300 mL (0.36 gal) 10-34-0 inorganic fertilizer to stimulate and maintain algae and zooplankton production. Ponds were sampled at night with lights and small nets, but low survival rates prevented harvesting of sufficient numbers to repeat the Year 1 experiment. Both ponds were harvested after 9 weeks yielding approximately 5,500 fry with an average mean weight of 1.7 g (0.06 oz). These fry were used in a habituation study, to examine density effect of conversion to dry feed and survival rates.

Yellow perch fry with an initial mean weight of 1.7 g (0.06 oz) were stocked at four densities (7.7, 11.5, 15.4, 18.5 g/L) into 25-L (6.6-gal) round fiberglass tanks at 21C (69.8F) and were initially fed Zeiglers Brothers crumbles #2 via automatic feeders at a rate of 7% body weight . Water flow was maintained high enough to ensure good water quality and adequate dissolved oxygen levels. The yellow perch fry had been starved for one week prior to the start of the feeding trial which lasted for 28 days. During this time, almost no mortalities were observed in any of the tanks and growth rates were not significantly different for the four densities.

OBJECTIVE 2

The principal accomplishments for this project were introduction of yellow perch aquaculture to three new aquaculturists, and identification of feeds that when fed to advanced fingerlings results in maximum weight gain.

The UNL focus on this objective, in cooperation with Pleasant Valley Fish Farm, was to evaluate under commercial conditions the feasibility of rearing age-1 yellow perch fingerlings to market size in aerated ponds supplied with groundwater to maintain water quality and moderate pond water temperature. As noted under Objective 1, spring and early summer in Nebraska in 1995 were unusually cold. The cold period was followed by very hot weather from midsummer through early autumn. Because of these climatic extremes plus some marketing decisions by Pleasant Valley Fish Farm, a decision was made to defer harvest of these production ponds from mid-October to mid-November

So, as with Objective 1, specific numerical findings on growth and other production data are not yet available for this report. One major change in procedure from Year 1 was that instead of comparing two sinking diets (one formulated specifically for perch by Purdue University versus Sterling Silver Cup trout feed), a single floating trout feed (Sterling Silver Cup) was evaluated. Visual observations to date indicate that perch can be readily trained to a floating feed and appear to grow as well or better on it than on nutritionally similar sinking diets. Regardless, the field trials conducted at Pleasant Valley Fish Farm unequivocally confirm that yellow perch can be reared from egg to market size within two growing seasons - a time frame similar to catfish production in the South.

Established producers in Nebraska and new producers in Indiana participated in an evaluation of feeds for yellow perch. Those feeds were from established fish feed companies or feed mills located in the region exploring the possibility of expanding their product line. Producers in both states reported fish fed diets from the established feed mills grew better and converted feed more efficiently than fish fed diets from local mills. Additional research is underway with local mills to help them upgrade their manufacturing capabilities to meet the needs of the developing perch industry.

A controlled study was conducted at Purdue University in which advanced fingerling fish (mean average weight = 50 g; 1.76 oz) were fed a series of experimental and commercial diets. Two experimental diets were identified that, when fed to fish, resulted in weight gains of 84 or 103% of the weight gain of fish fed the best commercial diet. Several commercial diets were fed to perch in the same study, four of which were formulated to meet the requirements of rainbow trout. Fish fed one of the trout diets gained 63% more weight over the course of the study than fish fed the poorest trout diet. Fish fed either of two types of diets formulated for catfish grew significantly worse than fish fed the trout diets.

WORK PLANNED

A NCRAC grant entitled Advancement of Yellow Perch Aquaculture has been funded for the 95-97 biennium. Objectives of this project are designed to continue work to improve larval rearing techniques by developing and evaluating starter diets in relation to size at transfer to formulated feeds under selected environmental conditions, improve pond fingerling production through examination of in-pond feeding using physical/chemical attractants and improved harvesting strategies for different sized fingerlings from various types and sizes of ponds, and develop extension materials and workshops emphasizing techniques. However, work on this project will continue as described below.

OBJECTIVE 1

UW-Milwaukee will conduct a workshop that demonstrates the intensive culture of yellow perch fingerlings using a recirculating system and establish the production costs for this type of rearing unit. Publications describing all completed studies are currently being prepared. UW-Madison researchers will continue to study the production of feed-trained and advanced yellow perch fingerlings using in-pond feeding techniques as detailed under Objective 2 of the NCRAC grant funded for the 95-97 biennium entitled Advancement of Yellow Perch Aquaculture.

The analysis of the data collected by UNL investigators from the field trials on yellow perch fingerling production conducted at Pleasant Valley Fish Farm will be completed and the findings submitted for publication, probably as a communication or note in The Progressive Fish-Culturist, or possibly as a NCRAC technical bulletin.

OBJECTIVE 2

The UNL field trials at Pleasant Valley Fish Farm on the production of food-size yellow perch will be completed in mid November 1995; the fish will be harvested for sale in Wisconsin; the production data analyzed by UNL investigators, and the findings submitted for publication, probably as a communication or note in The Progressive Fish-Culturist, or possibly as a NCRAC technical bulletin.

IMPACTS

The preparation of a summary of production cost information by UW-Milwaukee investigators for the intensive culture of yellow perch fingerlings in tanks will provide the necessary framework for writing a business plan for private sector producers who intend on using this fingerling production strategy. In addition, the continual refinement of intensive fry culture protocols will improve the production efficiency of this method. Information was developed on the physical, chemical and biological aspects of yellow perch production using RAS technology.

Studies on pond fingerling production by UW-Madison, UNL, and OSU researchers have shown that research-based production strategies can be used on a commercial scale to produce large numbers of perch fingerlings at a relatively low cost. The most promising of these strategies include the use of high fry stocking densities coupled with either (1) early pond harvest, for the subsequent habituation of fingerlings to formulated feeds in tanks; or (2) systems using lights and automatic feeders for habituating fingerlings to formulated feeds while they remain in ponds.

Studies at OSU have shown that pond produced yellow perch fry of 15-17 mm (0.59-0.67 in) in total length can be effectively weaned to practical trout diets at water temperatures of 21C (69.8F). Larger yellow perch fry (1.5-2.0 g; 0.05-0.07 oz) can also be weaned to trout diets following an initial period of starvation. This latter procedure reduces the mortalities due to stress and handling at a small size and allows for net harvesting or drain harvest of ponds.

The Nebraska field trials done collaboratively by the UNL and Pleasant Valley Fish Farm have clearly demonstrated that procedures developed or derived from earlier research projects can be utilized under commercial production conditions. Pleasant Valley Fish Farm, a major regional producer of yellow perch fingerlings, has benefited directly from new knowledge gained by participating in a NCRAC Yellow Perch Work Group project. At least one new fish farmer in Nebraska has begun producing food-size perch and is presently exploring market opportunities. Recently, a long-established Nebraska fish farmer has significantly increased his emphasis on the production of yellow perch. The number of inquiries about yellow perch aquaculture from Nebraskans and others in the NCR and Canada has increased noticeably in the past year.

The results of yellow perch research funded by NCRAC was presented at a workshop hosted at Spring Lake, Michigan in June of 1995. Presentations were made by four NCRAC researchers and two cooperators. The workshop was attend by over 50 prospective perch culturists from five States and one Canadian Province.

PUBLICATIONS, MANUSCRIPTS, AND PAPERS PRESENTED

See Appendix.SUPPORT

YEARS NCRAC-USDA FUNDING OTHER SUPPORT TOTAL

SUPPORT

UNIVER-SITY INDUSTRY OTHER

FEDERAL

OTHER TOTAL
1993-94 $75,000 $87,240 $30,000 $10,000a   $127,240 $202,240
1994-95 $75,000 $81,587 $30,000 $81,000abc   $192,587 $267,587
TOTAL $150,000 $168,827 $60,000 $91,000   $319,827 $469,827

aWisconsin Sea Grant/USDC/NOAA

bUSDI, Bureau of Indian Affairs

cUSEPA

HYBRID STRIPED BASS

Progress Report for the Period

September 1, 1993 to August 31, 1995

NCRAC FUNDING LEVEL: $168,000 (September 1, 1993 to August 31, 1995)

PARTICIPANTS:

Fred P. Binkowski University of Wisconsin-Milwaukee Wisconsin
George G. Brown Iowa State University Iowa
Paul B. Brown Purdue University Indiana
Konrad Dabrowski Ohio State University Ohio
James E. Ebeling Ohio State University Ohio
Terrence B. Kayes University of Nebraska-Lincoln Nebraska
Christopher C. Kohler Southern Illinois University-Carbondale Illinois
Jeffrey A. Malison University of Wisconsin Wisconsin
Robert J. Sheehan Southern Illinois University-Carbondale Illinois
Bruce L. Tetzlaff Southern Illinois University-Carbondale Illinois
R. Melvin White Purdue University Indiana
Extension Liaison:    
Joseph E. Morris Iowa State University Iowa
Non-Funded Collaborator:  
Fred Barrows U.S. Fish and Wildlife Service, Fish Technology Center, Bozeman Montana






PROJECT OBJECTIVES

(1) Develop larval diets and economically feasible techniques to convert hybrid striped bass young from zooplankton prepared diets.

(2) Develop intensive hatchery production techniques for white bass and to "domesticate" white bass by producing brood stock originating from induced spawns.

(3) Improve methods for storage and transport of striped bass and white bass gametes.

(4) Perfect cryopreservation techniques for white bass/striped bass semen and to demonstrate feasibility of hybrid striped bass production using "stored" semen in industry-type settings.

ANTICIPATED BENEFITS

The development of intensive larval culture techniques for white bass will allow for its full domestication, and will preclude the initial need for outdoor ponds. Because reciprocal cross hybrid striped bass are the same size as white bass at the swim-up stage, the results of this work will be directly applicable to their culture.

Conversion of larval fish to formulated feeds is one of the most difficult aspects of hybrid striped bass culture. Typically, high mortality and nonuniform acceptance of feed results. Thus, if flavor additives can be identified that entice consumption of feed, conversion to formulated feeds would be more uniform and lead to higher survival rates. Higher survival rates would result in higher profits for aquaculturists.

Development of efficient and reliable techniques to store, cryopreserve, and transport gametes (eggs and sperm) would improve breeding and production capabilities for culture technology of hybrid striped bass. Specifically, the development of these techniques will allow: (1) a continuous supply of gametes, (2) year-round production, (3) facilitation of selective breeding, and (4) more efficient use of available gametes. Although such methods need to be perfected for both semen and eggs, it is more likely that studies on semen will result in rapid development of technology for use in the aquaculture industry.

By working closely with a commercial producer in the region, it is hoped to directly transfer the developed semen storage technologies to the private sector, as well as satisfy future research objectives. This work, coupled with the out-of-season spawning work being conducted in our region and elsewhere, should greatly assist commercial producers to economically produce their own seed stock. Commercial producers would only need to maintain female brood stock of one of the species used in the cross. Sperm from the other species could be obtained elsewhere, stored until needed, and then used.

PROGRESS AND PRINCIPAL ACCOMPLISHMENTS

Research at Purdue University was designed to formulate and mix dry dietary ingredients, and facilitate manufacturing small pellets with the help of colleagues at the U.S. Fish and Wildlife Service, Fish Technology Center, Bozeman, Montana. Three diets were formulated in the first year of this project that contained two distinctive flavor additives that would be considered legal to use. That task was accomplished and diets were sent to colleagues at Southern Illinois University-Carbondale (SIU-C) and the University of Wisconsin-Milwaukee (UW-Milwaukee).

In a comparative study conducted at SIU-C, hatching rates for embryos incubated in Heath trays (28.2%) were equivalent to tannic acid-treated (150 mg/L water) embryos incubated in Heath trays (22.9%) or McDonald jars (22.4%).

Facilities to intensively rear larval white bass were established at Ohio State University (OSU) , SIU-C, and UW-Milwaukee. White bass larvae from three separate spawning trials were shipped by overnight freight to OSU and UW-Milwaukee. Attempts to rear larval white bass were minimally successful. Less than 1% survival rates were obtained by day 122 at UW-Milwaukee, day 45 at OSU and day 24 at SIU-C.

A group of white bass sac-fry shipped from SIU-C to UW-Milwaukee was introduced evenly by volume into twelve 60-L (15.8 gal) flow-through aquaria. Each aquarium contained approximately 300 sac-fry. These fish were offered "green tank" water and the three experimental diets that were provided by Purdue University. The length of the cylindrical food particles ranged from approximately 0.5 mm to 1.7 mm (0.02-0.07 in) and the diameter was 420-595 m. White bass sac-fry are approximately 3.5 mm (0.14 in) in total length. The cross sectional diameter of the feed approximated the width of the entire head (550-630 m) of white bass sac-fry, and was outside of the range of the width of the mouth. UW-Milwaukee researchers ground portions of the diets in a mortar and pestle and sieved it through a 150 m mesh to obtain more suitable-size particles. From May 26-31, 1995, each of the three ground and sieved diets was offered to fry in triplicate aquaria along with "green tank" water. The controls received only "green tank" water. No feeding activity or interest by the fry in the formulated diets was observed. Mortality of the sac fry was heavy in all the tanks and by May 31 (within 6 days), less than a dozen fry were observed in any of the aquaria and more than half of them had only one or no living fry. At this point the trial was terminated.

Past studies at Iowa State University (ISU) and SIU-C have allowed for evaluations of a number of semen extender and cryoprotectant solutions, and freezing and thawing methods. It was found that cryopreserved sperm showed promise for providing a cost-effective method for striped bass culturists to obtain seed stock. Studies at SIU-C showed that good fertility can be achieved in white bass eggs using cryopreserved spermatozoa.

Average fertility in several tests using white bass eggs fertilized with cryopreserved white bass sperm ranged from 22 to 48% of fertility with fresh, control semen. However, fertility was highly variable, and considerable motility was lost upon thawing frozen spermatozoa. Results with frozen striped bass spermatozoa and white bass eggs were better, but were also variable; average fertility for frozen striped bass spermatozoa ranged from 45 to 100% of control values.

Studies of sperm morphology at ISU indicated that some cryopreserved seminal samples (about 20% of those evaluated) showed clumping. Samples which exhibited clumping and adhesion showed no motility upon thawing, whereas samples where sperm morphology was normal and no clumping occurred became motile upon thawing. These results could explain much of the variability that has been observed in fertility tests, but it cannot be explained at this time why some samples undergo these adverse changes while others do not.

Studies at ISU also showed that best motility was routinely obtained when samples were activated with water prior to being completely thawed. This agrees with the results of fertility tests conducted at SIU-C; better fertility has routinely been obtained when cryopreserved semen is only partially thawed when combined with eggs.

In summer, 1995, Mississippi Fisheries, Inc. in Greenville, Mississippi, had a complete hybrid striped bass production failure, apparently due to the use of a new disinfectant. This company was supplying a major portion of the hybrid striped bass fingerling industry. Aquafutures, Inc., a Boston firm which annually raises over 1 million pounds of food-size hybrids, was not able to obtain sufficient numbers of fingerlings this year. In July 1995, a multi-state, interregional collaboration was initiated among SIU-C, University of Maryland and Aquafutures, Inc. SIU-C shipped white bass semen in extender solution to Dr. Zohar, University of Maryland. Dr. Zohar successfully induced a female striped bass to spawn and several million embryos were produced. Fertilization was over 70%. However, due to a severe heat wave which hit Baltimore two days later, all developing embryos were lost. Had fry been produced, Keo Fish Farm, in Keo, Arkansas, had agreed to rear the fry to fingerlings, and then to ship them to Aquafutures, Inc. An additional attempt will be made to accomplish this goal in November 1995. Aquafutures, Inc. is currently making arrangements with Florida producers to rear the larvae to fingerling size. This project clearly demonstrates the advantages of photothermal manipulation of spawning time in fishes, as well as the use of stored semen. Had it not been for an untimely heat wave, a major inter-regional, university/industry success story would have occurred.

WORK PLANNED

The last spawning/larval rearing trials will be conducted in November 1995. A hybrid striped bass workshop is planned for November 4, 1995, at Champaign, Illinois. A termination report for these project objectives will be submitted in winter, 1996.

IMPACTS

The potential impacts of intensive larval rearing and larval diet research are increased profitability. If flavor additives can be identified, a higher percentage of larvae can be trained to accept the feed and more juveniles can enter the food fish production cycle.

Related to this domestication of brood stock, is the availability of suitable gametes for successful fish reproduction. Because striped bass are typically difficult to obtain, it would be highly advantageous for the aquaculturist to have access to gametes without the difficulty of collecting or transporting the parent fish. The successful induction of white bass spawns and subsequent storage and transportation of Morone species gametes should go far in advancing the hybrid striped bass industry in the North Central Region. These technological advancements, combined with the cooperation of a regional commercial producer, will be transferred to the private sector in the form of fact sheets, videos, and workshops.



PUBLICATIONS, MANUSCRIPTS, AND PAPERS PRESENTED

See Appendix.

SUPPORT

YEARS NCRAC-USDA FUNDING OTHER SUPPORT TOTAL

SUPPORT

UNIVER-SITY INDUSTRY OTHER

FEDERAL

OTHER TOTAL
1993-94 $81,000 $58,679       $58,679 $139,679
1994-95 $87,000 $60,761       $60,761 $147,761
TOTAL $168,000 $119,440       $119,440 $287,440

WALLEYE

Progress Component Termination Report for the Period

September 1, 1989 to August 31, 1993

NCRAC FUNDING LEVEL: $321,740 (May 1, 1989 to August 31, 1993)

PARTICIPANTS: